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neweastbio@126.com
| 027-87561033
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| 價格 | 1600.00 |
| 品牌 | NewEast Biosciences  |
| 產(chǎn)地 | 中國 武漢 |
| 貨號 | 26052 |
| 規(guī)格 | 100μl |
| 說明書 | 說明書下載 |
EYA2 [26052]
[100 µL]
| Catalog Number: 26052 |
| Gene Symbol: EYA2 |
| Description: Anti- EYA2 Mouse Monoclonal Antibody |
| Background: EYA2 (eyes absent homolog 2) functions as a tyrosine phosphatase that specifically dephosphorylates Tyr-142 of histone H2AX. EYA2 promotes efficient DNA repair and coactivates SIX1. Together with SIX1 and DACH2, EYA2 seems to be involved in myogenesis. |
| Immunogen: A synthetic peptide near the N-terminus of human EYA2. |
| Applications: ELISA, WB, IHC, IP |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti EYA2 antibody recognizes EYA2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Western blot: |
 |
Western blot analysis of recombinant full-length EYA2 protein. Purified His-tagged EYA2 protein (Cat. # 10195) was blotted with anti EYA2 monoclonal antibody (Cat. # 26052). |
| Immunohistochemistry: |
|
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma tissue with anti EYA2 monoclonal antibody (Cat. # 26052). Tissue section was fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody. |
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