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價(jià)格 | ¥4800.00 |
品牌 | NewEast Biosciences |
產(chǎn)地 | 中國(guó) 武漢 |
貨號(hào) | 26080 |
規(guī)格 | 100μl |
說(shuō)明書(shū) | 說(shuō)明書(shū)下載 |
ErbB2(V777A) [26080]
[100 µL]
Catalog Number: 26080 |
Gene Symbol: ERBB2/HER2/Neu |
Description: Anti ErbB2(V777A) mouse monoclonal Antibody |
Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers. |
Immunogen: A synthetic peptide surround the 777A codon of ErbB2 protein. |
Tested Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IF: 1:100 IHC: 1:50-1:100 |
Concentration: 0.2 mg/ml |
Host: mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
Immunofluorescence: |
Immunofluorescence of cells expressing ErbB2 proteins with Anti ErbB2(V777A) antibody. HEK293T cells were transfected with pCDNA3-GFP ErbB2 WT plasmid (left column) or pCDNA3-GFP ErbB2(V777A) plasmid (right column), then fixed and stained with Anti ErbB2(V777A) monoclonal antibody (Cat. #26080). |
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