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neweastbio@126.com
| 027-87561033
PRODUCT CENTER
| 價(jià)格 | ￥4800.00 |
| 品牌 | NewEast Biosciences  |
| 產(chǎn)地 | 中國(guó) 武漢 |
| 貨號(hào) | 26176 |
| 規(guī)格 | 100ul |
| 說(shuō)明書 | 說(shuō)明書下載 |
EGFR(vIII) [26176]
[100 µL]
| Catalog Number: 26176 |
| Gene Symbol: EGFR/HER1/ERBB1 |
| Description: Anti EGFR(vIII) Mouse Monoclonal Antibody |
| Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC). |
| Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes EGFR(vIII), but not wild type EGFR from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant EGFR(vIII) and wildtype proteins. Purified His-tagged EGFR(vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR(vIII) mouse monoclonal antibody (Cat. # 26176). |
| Immunofluorescence: |
|
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(vIII) plasmid (right column), then fixed and stained with anti EGFR(vIII) monoclonal antibody (Cat. #26176). |
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| Catalog# | Name | Size | Price |
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| 26115 | Anti EGFR(G719A) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
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| 26121 | Anti EGFR(V769L) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
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| 26116 | Anti EGFR(N826S) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26176 | Anti EGFR(vIII) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26221 | Anti EGFR(L858Q) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26481 | Anti EGFR(T854A) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| 26483 | Anti EGFR(S768I) Mouse Monoclonal Antibody | 100 μL | ￥4800 |
| Publications: |
| 1. Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma Tumor Biology. October 2014 |
發(fā)表時(shí)間：2021
名稱/貨號(hào)：Anti- EGFR(vIII) Mouse Monoclonal Antibody/26176
影響因子：Mol Cancer Ther IF=5.3
應(yīng)用范圍：IHC
種屬：Human
技術(shù)支持：優(yōu)狐
